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Excitation and emission fluorescence were spectrally separated using a dichroic, passed through a single band filter, and focused onto a photodetector to record real-time VTA activity as animals socialized jannacci paolo allegra a novel conspecific in their home cage (Fig. 1A). There was a marked increase in VTA GCaMP signal paool the test jznnacci investigated the conspecific, specifically time-locked to appetitive approach and contact lallegra compagnia lamezia terme of the other mouse, which habituated over time (Fig. 1B). These data show for the first time the real-time dynamics of VTA DA neurons during a complex social behavior, and although correlative, they suggested that experimentally prolonging these habituating VTA signals might be a way to increase the animal's social behavior. Optical recording of dopaminergic dynamics during social interaction. ( ALeft ) Fiber photometry setup. Light path for GCaMP fluorescence excitation and emission is through a single optical fiber implanted in the VTA. ( Right ) viral targeting of GCaMP5 to VTA DA neurons.

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